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[目的]研究低温处理对紫苏生理指标的影响,确定造成紫苏叶片低温伤害的时间。[方法]将紫苏叶分别低温处理0、12、24、36、48和60 h,并测定其叶绿素、可溶性蛋白质及可溶性糖的含量,并考察2~5℃低温对紫苏叶片生理指标的影响,确定紫苏叶片低温伤害的时间。[结果]低温处理48 h能明显减少叶片中叶绿素的含量,而且低温处理60 h的叶绿素含量减少非常明显;低温处理48 h能明显减少叶片中可溶性蛋白质和可溶性糖的含量。[结论]低温处理超过48 h,紫苏就会受到伤害;低温处理时间达到60 h时,紫苏受到的伤害程度加重。 相似文献
53.
以食品中分离到的L.mXFL0605株为试验菌株,PCR扩增得到iap全基因。将iap连接到pET-28a(+)载体进行原核表达,分别采用培养温度为25℃或37℃,IPTG浓度为5μg/mL或10μg/mL优化组合进行诱导。结果显示,当采用37℃培养和10μg/mLIPTG诱导时可以获得部分可溶性表达的p60,重组p60蛋白分子质量约60 ku。 相似文献
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建立大豆水溶性蛋白含量测定(NY/T 1205-2006)不确定度评定的数学模型,对检测过程中带来的各个不确定度分量进行识别和量化,提出应用该方法检测的不确定度评定结果。 相似文献
56.
丽蚜小蜂Encarsia formosa Gahan是粉虱类害虫的重要寄生性天敌昆虫,补充糖分等营养可以延长丽蚜小蜂寿命及增加其产卵量,但对其糖转运相关蛋白基因及其糖诱导表达尚不清楚。本研究克隆获得丽蚜小蜂的糖转运蛋白EfST1基因,与其他寄生蜂的糖转运蛋白基因序列同源性达65%以上;进一步采用实时荧光定量qPCR技术检测发现,饲喂不同浓度的葡萄糖溶液均可显著提高EfST1基因的表达量,且饲喂不同时间段(2~48 h)后,丽蚜小蜂EfST1基因表达量均显著高于对照组,2 h和48 h处理组间差异不显著。综上,EfST1基因可能与糖分补充及其转运密切相关,该基因诱导表达受糖分浓度影响小。研究结果为进一步研究寄生蜂生物防治过程中补充营养及其转运机制奠定基础。 相似文献
57.
《Communications in Soil Science and Plant Analysis》2012,43(15):1789-1796
In the Red River Valley, about 385,000 Mg of lime is used to help refine about 39,000 Mg of sugar beets. In a greenhouse study, sugar-beet-processing lime (spent lime; SL), commercial ag lime (AL), and reagent-grade lime (RL) were added to an acidic soil at rates of 0, 1.96, 3.93, and 7.86 Mg ECC ha?1. Wheat was planted, and aboveground biomass and root masses were determined after 44 d of growth. Soil pH increased the greatest with the highest lime application rates. Incorporation of lime uniformly increased soil pH in the pots, whereas with surface application, soil pH decreased with increasing depth. The SL yielded more aboveground soil biomass and root mass than the AL and RL. Use of SL for increasing soil pH and reducing exchangeable Al in acid soils would be a viable use for this industrial by-product. 相似文献
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Olga E. Scholten Lee W. Panella Theo S.M. De Bock Wouter Lange 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(2):161-166
Rhizoctonia solani Kühn is a serious plant pathogenic fungus, causing various types of damage to sugar beet (Beta vulgaris L.). In Europe, the disease is spreading and becoming a threat for the growing of this crop. Plant resistance seems to be the most practical and economical way to control the disease. Experiments were carried out to optimise a greenhouse procedure to screen plants of sugar beet for resistance to R. solani. In the first experiment, two susceptible accessions were evaluated for root and leaf symptoms, after being grown in seven different soil mixtures and inoculated with R. solani. The fungus infected all plants. It was concluded that leaf symptoms were not reliable for the rating of disease severity. Statistically significant differences between the soil mixtures were observed, and there were no significant differences between the two accessions. The two soil mixtures, showing the most severe disease symptoms, were selected for a second experiment, including both resistant and susceptible accessions. As in the first experiment, root symptoms were recorded using a 1–7 scale, and a significant expression of resistance was observed. The average severity of the disease in the greenhouse experiment generally was comparable with the infection in field experiments, and the ranking of the accessions was the same in the two types of experiments. It was concluded that evaluation procedures in the greenhouse could be used as a rapid assay to screen sugar beet plants for resistance to R. solani. 相似文献
60.
H. G. Smith I. Barker G. Brewer M. Stevens P. B. Hallsworth 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(2):163-169
A sugar-beet-infecting isolate of beet mild yellowing luteovirus (BMYV), and aBrassica-infecting isolate of beet western yellows luteovirus (BWYV) were used to produce monoclonal antibodies for epidemiological studies with BMYV and related field strains. Thirty-four monoclonal antibodies were tested for their reaction with 9 luteoviruses in triple-antibody-sandwich enzyme-linked immunosorbent assay. One (MAFF 24) is now routinely used in the UK for detecting BMYV and BWYV in plants and aphids, although it does not discriminate between them. Heterologous reactions were detected between some of the monoclonal antibodies and potato leafroll virus (PLRV), bean leafroll virus (BLRV) and barley yellow dwarf virus (BYDV-RPV). 38% of antibodies raised to BWYV reacted with PLRV compared with 4% of those raised to BMYV. Monoclonal antibodies were produced which distinguished a sugar-beet-infecting isolate of BMYV with differing host range and serological properties from the commonly-occurring field strain. 相似文献